IL-18 IFN-γ expression in labial glands and blood of patients with primary Sj(o)ren's syndrome
10.3760/cma.j.issn.1007-7480.2012.09.011
- VernacularTitle:白细胞介素-18及干扰素-γ在原发性干燥综合征患者唇腺组织和外周血中的表达
- Author:
Kexia CHAI
;
Shenglan WANG
- Publication Type:Journal Article
- Keywords:
Sj(o)gren's syndrom;
Immunohistochemistry;
Labial gland
- From:
Chinese Journal of Rheumatology
2012;16(9):625-628
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThis study explored the change of IL-18,and IFN-γ and their role in the pathogenesis of primary Sj(o)gren's syndrome (pSS) by determining the expression of IL-18,IFN-γ in labial glands and blood of patients with pSS.MethodsThe levels of IL-18,IFN-γ in labial glands of patients with pSS were determined by immunohistochemical method.The level of serum IL-18 was determined with enzyme linked immunosorbent assay ELISA.The level of γ globulin was measured by the cellulose acetate membrane method.SPSS 17.0 statistical software was used for data analysis.The t test,Fisher's exact probability test and index correlation analysis were performed with linear correlation analysis.Spearman's test was used to examine the association between the expression of IL-18 and IFN.ResultsThe positive rate of IL-18 and IFN-γ in patients with pSS was much higher than normal control group(P＜0.05).And the IL-18 and IFN-γ levels were positively correlated withpatients with pSS (P＜0.05).The level of serum IL-18 in patients with pSS was (216±85) pg/ml,which was much higher than normal control group(39±8) pg/ml.The IL-18 level and γ globulin(23.2±2.6)％ was positively correlated in patients with pSS (r=0.538,P＜0.05).ConclusionThe expression of IL-18 and IFN-γ in labial glands of patients with pSS are much higher in patients with pSS than those of the normal controls.Theresults have shown that IL-18 and IFN-γ may be involved inthe pathogenesis of pSS.
