Radioimmunoimaging and biodistribution of 131I-Herceptin in breast cancer xenograft BALB/c-neu mousse
10.3760/cma.j.issn.1007-631X.2012.05.017
- VernacularTitle:131I-Herceptin在乳腺癌裸鼠模型中的显像及体内分布研究
- Author:
Zhixue YANG
;
Shaohua WEI
;
Guoqin JIANG
;
Zengli LIU
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Receptor,epidermal growth factor;
Antibodies,monoclonal;
Isotope labeling;
Radionuclide imaging;
Mice
- From:
Chinese Journal of General Surgery
2012;27(5):402-405
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the biologic distribution of 131I-Herceptin in BALB/c-neu nude mice bearing HER-2 positive SK-BR-3 human breast cancer xenografts and the radioimmunoimaging characteristics of nude mouse bearing human SK-BR-3 breast cancer xenografts. MethodSK-BR-3 breast cancer cells were implanted subcutaneously to athymic mice to establish animal model.Tumor bearing mice were continuously imaged with SPECT. The radiocounting per minute (cpm) of different organ on a γ-arithmometer was measured at 4,12,24,48 h postinjection of 131I-Herceptin or 131I-mlgG,and the T/NT ratios and the uptake percentages per gram of the injection dose (％ ID/g) was gained. ResultsModel was established in 96％ nude mouse.Compared with the control group,there was a significantly stronger contrast enhancement of tumor imaging,bigger T/NT and ％ ID/g in experimental group ( P ＜ 0.0l ).Conclusions 131I-Herceptin concentrates obviously in implanting tumor tissues of nude mouse,hence it is a good radiopharmaceutical agent targeting SK-BR-3 xenografts.
