Bone augmentation in an osteoporosis rat model via a single local injection of simvastatin
10.3760/cma.j.issn.0253-2352.2012.05.022
- VernacularTitle:骨质疏松大鼠股骨内单次注射辛伐他汀强化骨骼的实验研究
- Author:
Ning YANG
;
Yueyi CUI
;
Xiaoguang HAN
;
Huijie LENG
;
Zhongqiang CHEN
;
Zhongjun LIU
;
Gengting DANG
;
Chunli SONG
- Publication Type:Journal Article
- Keywords:
Osteoporosis;
Administration,topical;
Osteogenesis
- From:
Chinese Journal of Orthopaedics
2012;32(5):500-506
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the bone anabolic effects after a single local injection of simvastatin into femoral cavities of osteoporotic rats.MethodsThirty-six female SD rats(3 months old,body weight 250-300 g) were ovariectomized(OVX) and low-calcium-diet fed for 3 months,OVX rats were randomized into 3 groups(n=12).Left femurs of group A,B and C were injected with 0,5 and 10 mg simvastatin,respectively.Half of the rats in each group were randomly euthanized separately 1 and 5 months after simvastatin injection.Left femurs were taken out for bone mineral density (BMD) assessment with dual energy X-ray absorptiometry,bone histomorphometic changes were analysized by Micro-CT,and two kinds of biomechanical tests were used to evaluate the osteogenic effects.ResultsOne and five months after injection,BMD in mid-diaphysis significantly increased in simvastatin-injected groups compared to the control group.For Micro-CT analysis,significant increase in total bone volume/total tissue volume,cortical wall thickness,trabecular thickness,trabecular number,and a significant decrease in trabecular spacing were observed in simvastatin-injected groups compared to the control group.For both biomechanics (the three-pointbreaking test of condyles and axial compressive testing of proximal femur),the values were significantly higher in simvastatin-injected groups than the control group.ConclusionLocal simvastatin treatment showed a positive effect on improving mechanical strength,structure of osteopenic femurs and BMD.Our findings may provide a new strategy for the prevention and treatment of osteoporosis,especially for osteoporotic fractures.
