Changes of DRAM expression in radiation-induced autophagy in breast cancer cells
10.3760/cma.j.issn.0254-5098.2012.01.003
- VernacularTitle:电离辐射诱导乳腺癌细胞自噬相关基因DRAM表达的研究
- Author:
Zhiheng SONG
;
Xiaodong LIU
;
Huiying XU
;
Bing LIANG
;
Lili JIA
;
Dejuan KONG
;
Heqing YI
;
Mengzi HE
;
Shumei MA
- Publication Type:Journal Article
- Keywords:
Autophagy;
X-rays;
DRAM gene
- From:
Chinese Journal of Radiological Medicine and Protection
2012;32(1):11-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of DRAM in breast cancer cell line MCF-7 in radiation-induced autophagy. Methods GFP-LC3 transfection method was used to observe autophagy bubble.Real time-PCR was used to detect DRAM and MAPLC3 from transcriptional and translational level,respectively. The silencing vector from gene engineering was introduced by calcium phosphate transfection.Results Compared with the control group,GFP-LC3 increased significantly after 8 Gy irradiation by immunofluorescent assay,and the level of MAP-LC3 expression was higher than control group after 8 Gy irradiation by Western blot ( F =5.38,8.72,10.63,15.23,20.78 and 55.23,P ＜ 0.05 ).DRAM protein expression increased significantly at 2 h in the 8 Gy time-dose study,up to maximum at the 32 h( F =116.34,P ＜ 0.05 ).In DRAM model,the expression of LC3 and DRAM decreased significantly (F =20.36 and 40.35,P ＜ 0.05 ) and DRAM expression increased 8 Gy post-irradiation,but still lower than that in 8 Gy irradiation wild-type group.The LC3 expression also decreasaed 8 Gy post-irradiation(F =50.34,P ＜ 0.05 ).Conclusions DRAM plays an important role in irradiation-induced autophagy in breast cancer cell.DRAM might participate in the process and serve as a theoretical target for clinical treatment of breast cancer.
