Expression of RUNX3 and miR-130b in gastric carcinoma and clinical significance
10.3760/cma.j.issn.1007-631X.2012.09.016
- VernacularTitle:miR-130b和RUNX3mRNA在胃癌组织中的表达及其临床意义
- Author:
Yuehan REN
;
Jixian CHEN
;
Dixin XUE
;
Hongmin YU
;
Weili WU
;
Renhu ZHANG
;
Daozhe LIN
;
Ming YU
;
Xiao LIN
;
Meizhen LIANG
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Micro RNAs;
Pathology,clinical;
Prognosis
- From:
Chinese Journal of General Surgery
2012;27(9):743-746
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the expression of miR-130b and RUNX3mRNA in human gastric carcinoma and the clinical significance. MethodsThe expression of miR-130b and RUNX3mRNA were detected by RT-PCR in 40 cases of gastric carcinoma and corresponding normal mucosa tissue. The expression of RUNX3protein was determined by immunohistochemistry SP method. ResultsThe expression of miR-130b was significantly up-regulated in gastric carcinoma than that in the adjacent normal gastric mucosa tissues (2.18 ± 3.75 ) vs.( 2.59 ± 3.45 ),P ＜ 0.05 ; The expression of RUNX3mRNA in gastric carcinoma tissues was significantly lower than that in adjacent normal gastric mucosa tissues( 8.76 ±2.82) vs.( 7.58 ± 2.87 ),P ＜ 0.05.The expression of miR-130b and RUNX3mRNA were positively correlated with lymph node metastasis and clinical stage ( P ＜ 0.05 ) ; No significant association was found between the expression and age,gender,tumor size,distant metastasis and depth of tumor invasion ( P ＞0.05 ).The expression of miR-130b was negatively correlated with RUNX3 protein expression in nuclei and cytoplasm (P ＜ 0.05 ).ConclusionsAbnormal expression of miR-130b and RUNX3mRNA correlates with prognosis of gastric carcinoma; Decreased RUNX3 protein expression may contribute to tumourigenesis.
