Endocytosis and exocytosis of gold nanochain attaching Hep-2 cells of human laryngeal carcinoma
10.3969/j.issn.1673-8225.2012.08.008
- VernacularTitle:金纳米链与人喉癌Hep-2细胞共培养自由进出细胞的形式
- Author:
Linhai CONG
;
Xiaoguang HE
;
Yibing YANG
;
Shiwen ZHANG
;
Shukun PENG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2012;16(8):1363-1366
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: The gold nanoparticles have a killing effect on tumor cells.OBJECTIVE: To investigate the influence of gold nanochain on Hep-2 cells proliferation of human laryngeal carcinoma.METHODS: The gold nanochain was prepared by a glucose synthesis method and added into the culture cells with different concentrations (10%, 25%, 50%, 75%, 95%) to test the influence on proliferation of in vitro cultured Hep-2 cells. The endocytosis and exocytosis of transmembrane when gold nanochain attached to Hep-2 cells were observed by electron microscopy.RESULTS AND CONCLUSION: The gold chain at high concentrations (75%, 95%) exhibited inhibitory effects on the proliferation of Hep-2 cells, but the influence was not increased with increasing concentration, belonging to a range of non-toxic. Gold nanchain can enter into Hep-2 cells after 8 hours of co-culture and leave cells after 48 hours, indicating gold nanoparticles chain can enter and leave Hep-2 freely.
