Subcutaneous implantation of bone marrow mesenchymal stem cells-polyglycolic acid scaffold complex to construct small diameter tissue-engineered blood vessels
10.3969/j.issn.1673-8225.2011.51.011
- VernacularTitle:骨髓间充质干细胞-聚乙醇酸支架复合体种植动物皮下构建组织工程化小口径血管
- Author:
Guoliang GAO
;
Jianfei SONG
;
Haiyong WANG
;
Min ZHENG
;
Wei WANG
;
Yiyao JIANG
;
Zhenzong DU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2011;15(51):9544-9548
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Our former studies have shown that bone marrow mesenchymal stem cells (BMMSCs) can be induced differentiation to vascular smooth muscle-like cells (VSMLCs) and vascular endothelium-like cells (VELCs), which are compatible with collagen-embedded polyglycolic acid scaffolds. OBJECTIVE: To investigate the possibility of constructing small diameter tissue-engineered blood vessels via subcutaneous implantation. METHODS: The cells-scaffold complex was produced by separately seeding VSMLCs and VELCs derived from BMMSCs on polyglycolic acid collagen scaffolds. The two layers were separated by ECMgel. The cells-scaffold complex was subcutaneous implanted into small diameter tissue-engineered blood vessels.RESULTS AND CONCLUSION: Histological analysis of the small diameter tissue-engineered blood vessel walls revealed a typical artery structure, which was similar to natural vessels. The tissue-engineered blood vessels were not broken down under a force of 26.6 kPa. Eight weeks after implantation, the Brdu-labeled seed cells were found in the three layers of the vessel walls. The results revealed that the subcutaneous tissue was a good bioreactor to construct small diameter tissue-engineered blood vessels.
