pcDNA3.1-osteogenic growth polypeptide eukaryotic expression vector in bone marrow mesenchymal stem cells

VernacularTitle:pcDNA3.1-成骨生长肽真核表达载体在骨髓基质干细胞中的表达
Author: Gang AN ; Songcen Lü ; Yashan GUO ; Zhen XUE ; Qiukui DENG
Publication Type:Journal Article
From: Chinese Journal of Tissue Engineering Research 2011;15(36):6696-6700
CountryChina
Language:Chinese
Abstract: BACKGROUND: Osteogenic growth polypeptide (OGP) had clear effect on promoting osteoblast proliferation, differentiation and mature. OBJECTIVE: To explore the expression of OGP gene, which was transfected into rabbit bone marrow mesenchymal stem cells (BMSCs) and to evaluate the effects of OGP on differentiation of rabbit BMSCs. METHODS: pcDNA3.1-OGP was constructed using gene cloning and recombination techniques. Rabbit BMSCs were transfected with pcDNA3.1-OGP mediated by lipofectamine 2000. The transfection positive cell clones were selected with G418. The expression of OGP gene was detected using reverse transcription-polymerase chain reaction analysis on an mRNA level. Differentiation of pcDNA3.1-OGP transfected BMSCs into osteoblast lineage was observed. RESULTS AND CONCLUSION: The pcDNA3.1-OGP plasmid was constructed successful and OGP expression was detected in rabbit BMSCs. Hydroxyproline content was increased, and alkaline phosphatase activity was also increased. These indicate that pcDNA3.1-OGP transfected BMSCs expressed OGP, and could differentiate into osteoblast lineage.