Expression and biological function of miR-622 in gastric cancer cells

VernacularTitle:miR-622在胃癌细胞中的表达及其功能的研究
Author: Ruizheng MIAO ; Leping LI ; Jianfeng LI ; Meng WANG ; Hongguang SHENG ; Tao XU
Publication Type:Journal Article
Keywords: Stomach neoplams; MicmRNAs; Gene expression
From: Chinese Journal of General Surgery 2011;26(11):932-935
CountryChina
Language:Chinese
Abstract: Objective To investigate the biological function of miR-622 in human gastric cancer cell lines of SGC-7901 and NCI-N87 cells and its role in gastric carcinogenesis.Methods We analyzed the expression of miR-622 in those human gastric cancer cell lines by quantitative real-time polymerase chain reaction.Tumorigenesis,migration and invasion ability of miR-622 overexpression was assessed in vitro with miR-622 precursor and inhibitor in in SGC-7901 and NCI-N87 cells.Results The expression level of miR-622 in SGC 7901 was 1.29 ± 0.57,and it was 10.96 ± 1.02 in NCI-N87 cells.The soft agar colony formation rate was 76％ in SGC-7901 after transfecting miR-331-3p precursor,the ability of scratch healing was ( 11 ±7) μm,and the ability of transwell invasion was (731 ±3),compared with that in control group,the differences were statistically significant ( P ＜ 0.05 ).Conclusions Over-expression of miR-622 promotes tumorigenesis,migration,and invasion in gastric cancer cells in vitro.