Investigation on the effect of SaIB on bone marrow-derived mesenchymal stem cells apoptosis induced by hypoxia and serum deprivation
10.3760/cma.j.issn.1673-4246.2011.09.013
- VernacularTitle:丹酚酸B抗缺氧无血清诱导的大鼠骨髓间充质干细胞凋亡的研究
- Author:
Kai CHEN
;
Wei CAO
;
Ruixia XU
;
Xiaowen GUO
;
Jie WANG
- Publication Type:Journal Article
- Keywords:
SalB;
Bone marrow-derived Mesonchymal stem cells;
Hypoxia/SD;
Apoptosis
- From:
International Journal of Traditional Chinese Medicine
2011;33(9):807-810
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of SalB on bone marrow-derived mesenchymal stem cells (BMSCs) apoptosis induced by hypoxia and serum deprivation (hypoxia/SD) in the vitro. Methods BMSCs were cultured in the vitro and randomly divided into control group, hypoxia/SD group and SalB group.SalB group was composed by four groups and were pretreated by complete medium with 0.1、 1、 10、 100 mg/L SalB for 1 hour. And after that they were washed with phosphate buffer for 2 times, added by IMDM with 0.1、1、 10、 100 mg/L SalB and cultured with hypoxia/SD group together in the same condition of hypoxia/SD for 6hours. The control group was cultured for 6 hours in the condition of aerobic and enough serum. Apoptosis was detected by Hoechst33342 staining with inverted phase contrast, fluorescence microscope and Annexin V/PI dual-color flow cytometry. Results Significant apoptosis of BMSCs was induced by hypoxia/SD in the vitro.The early apoptosis of BMSCs induced by hypoxia/SD was significantly decreased by SalB of 0.1、 1、 10 mg/L(P＜0.05) . Conclusion0.1、 1、 10 mg/L SalB can decrease the early apoptosis of BMSCs induced by hypoxia/SD.
