Molecular mechanism of oncogenic B-RafV600E induces chromosome instability in melanoma Sbcl2 and SK-MEL31 cells
10.3760/cma.j.issn.1006-9801.2012.09.004
- VernacularTitle:癌基因B-RafV600E导致黑色素瘤Sbcl2和SK-MEL31细胞染色体不稳定性的分子机制研究
- Author:
Shujing LI
;
Yanyan ZHANG
;
Jing LIU
;
Ling ZHANG
;
Ruyi SHI
;
Zhiping ZHAO
;
Chanting HE
;
Xiaolong CHENG
- Publication Type:Journal Article
- Keywords:
Genes,B-RafV600E;
Genes,Mps1;
Centrosome;
Mitotic spindle apparatus;
Chromosome
- From:
Cancer Research and Clinic
2012;24(9):589-592
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the molecular mechanism of BRAFV600E inducing chromosome instability in Sbcl2 and SK-MEL31 melanoma cells.Methods The endogenous Mps1 in stable Sbcl2-and SK-MEL31-B-RafV600E expression cells were depleted by siRNA approach.To test the effect of B-RafV600E on the centrosome amplification and the formation of multipolar spindles,cells at S-phase with HU-treatment were arrested and then the centrosomes and mitotic spindles structure were detected through immunofluoresence.Results The percentage of B-RafV600E expressing Sbcl2 and SK-MEL31 cells (Sbcl2-B-RafV600E and SKMEL31-B-RafV600E) with centrosome amplification and multipolar spindle was reduced from 36 ％ to 6 ％ when Mps1 was absent.Conclusion B-RafV600E leads to centrosome amplification and multipolar spindle through Mps1,thus results in chromosome instability in Sbcl2 and SK-MEL31 melanoma cells.
