Effects of hydrogen gas on lipopolysaccharide-induced apoptosis in human umbilical vein endothelial cells in vitro
10.3760/cma.j.issn.0254-1416.2012.08.020
- VernacularTitle:氢气对脂多糖诱导人脐静脉内皮细胞凋亡的影响
- Author:
Huanzhi HAN
;
Keliang XIE
;
Hongguang CHEN
;
Daquan LIU
;
Lijun ZHANG
;
Yonghao YU
- Publication Type:Journal Article
- Keywords:
Hydrogen;
Lipopolysaccharides;
Umbilical veins;
Endothelial cells;
Apoptosis
- From:
Chinese Journal of Anesthesiology
2012;32(8):973-975
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hydrogen gas on lipopolysaccharide (LPS)-induced apoptosis in human umbilical vein endothelial cells (HUVECs) in vitro.Methods HUVEC-12 cells were seeded in 96-well plates with a density of 1 × 104/ml (200 μl/hole) or in 6-well plates (2 ml/hole) with a density of 1 × 106/ml and randomly divided into 4 groups (n =30 each):control group (group C),hydrogen gas (H2) group,LPS group and LPS + H2 group.The cells were cultured in the plain culture medium in groups C and LPS or in hydrogen-saturated culture medium in groups H2 and LPS + H2.In addition,LPS 1 μg/ml was added simultaneously in groups LPS and LPS + H2 and the equal volume of normal saline was added instead in groups C and H2.The cell viability and apoptosis were measured by MTT assay and flow cytometry respectively after 24 h incubation.The concentration of high-mobility group box 1 (HMGB1) in the supernatant was determined by ELISA.Results Compared with groups C and H2,the cell viability was significantly decreased,and the apoptotic rate and concentration of HMGB1 in the supernatant were significantly increased in groups LPS and LPS + H2 (P ＜ 0.05).Compared with group LPS,the cell viability was significantly increased,and the apoptotic rate and concentration of HMGB1 in the supernatant were significantly decreased in group LPS + H2 (P ＜ 0.05).Conclusion Hhydrogen gas can effectively reduce LPS-induced apoptosis in HUVECs through inhibiting the release of HMGB1.
