Hydrogen-rich saline alleviation on the oxidative stress and early-phase radiation-induced brain injury in rats
10.3760/cma.j.issn.0254-5098.2012.05.008
- VernacularTitle:饱和氢盐水减轻大鼠急性放射性脑损伤早期的氧化应激
- Author:
Hongmei HUO
;
Shun YANG
;
Liesong CHEN
;
Haijun Lü
;
Aidong WANG
;
Liyuan ZHANG
- Publication Type:Journal Article
- Keywords:
Hydrogen;
Irradiation;
Protection;
Brain injury;
Free radical
- From:
Chinese Journal of Radiological Medicine and Protection
2012;32(5):485-487
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hydrogen on radiation-induced acute injury in rat brain.Methods Forty-five mature Sprague-Dawley rats were randomly divided into three groups:saline therapy group,hydrogen therapy group and healthy control group.The whole brain of SD rat was irradiated with single dose of 20 Gy by 4 MeV electrons.Rats in therapy group were injected with hydrogen-rich saline after irradiation and were sacrificed at 1,3,7,14 d post-irradiation.The changes of malonaldehyde (MDA),superoxidase dismutase (SOD) and 8-hydroxydeoxygunosine (8-OHdG) in brain homogenate and the pathological changes in brain hippocampus were observed.Results The brain water content (t=3.78,3.18,P＜0.05) and the contents of 8-OHdG (t=2.33,2.71,2.33,P＜0.05) in the therapy group was lower than the control group at 7 d and 14 d post-irradiation.The contents of SOD were significantly higher(t =2.41-2.92,P ＜ 0.05) from 1 to 7 day,while the contents of MDA were significantly lower in therapy group than those in the control group from 1 to 14 day post-irradiation (t =4.01-6.20,P ＜ 0.05).Moreover,the damage degree in the nerve cells of hippocampus was less compared to the control group.Conclusions The hydrogen-rich saline could have protection role in irradiation-induced acute brain injury in rats.
