Expression and significance of Dysadherin mRNA in renal clear cell carcinoma
10.3760/cma.j.issn.1006-9801.2012.08.003
- VernacularTitle:Dysadherin mRNA在肾透明细胞癌中的表达及其意义
- Author:
Hong XIAO
;
Guiling FAN
;
Huixia ZHENG
;
Gang LIANG
;
Yanglu ZHAO
;
Ning LI
;
Caixia CHENG
;
Hongkun WANG
;
Jianfang LIANG
- Publication Type:Journal Article
- Keywords:
Kidney neoplasms;
Dysadherin;
Reverse transcriptase polymerase chain reaction;
Immunohistochemical
- From:
Cancer Research and Clinic
2012;24(8):512-514
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of Dysadherin and analyze its role in renal clear cell carcinoma (RCCC).Methods RT-PCR and immunohistochemical were used to detect the expression of Dysadherin in 60 cases of fresh RCCC and 60 adjacent normal renal tissues(male 35,female 25; age 37-78,median age 61; ＞7 cm 24,≤7 cm 36; Ⅰ/Ⅱ 39,Ⅲ/Ⅳ 21).Results Dysadherin mRNA expression in RCCC tissues (2.0043±0.2890) was higher than that in adjacent normal renal tissues (0.8461 ±0.2479) (t =6.8020,P ＜ 0.05).Dysadherin expression was associated with nuclear grade.The expression of Dysadherin in nucleus grade Ⅲ and Ⅳ tumors were significantly higher than that in nucleus grade Ⅰ and Ⅱ tumors [the mRNA expression were 4.6224±0.3194,2.7780±0.2288,the positive rates of protein were 64.1％ (25/39),95.2 ％ (20/21) (t =6.5750,x2 =5.495,P ＜ 0.05)].There was no association between the expression of Dysadherin with sex (t =1.0530,x2 =0.023),age(t =0.0511,x2 =0.089) and tumor size (t =1.0330,x2 =0.370) (P ＞ 0.05).Conclusion In RCCC,Dysadherin expression is positively associated with tumor aggressiveness based on grading.It seems that Dysadherin may be a valuable prognostic marker in RCCC.
