Influence of passive smoking on expression of TGF-β1 and mutation of p53 gene in rat lung tissues
10.3760/cma.j.issn.1006-9801.2011.11.008
- VernacularTitle:被动吸烟对大鼠肺组织转化生长因子β1表达和p53基因突变的影响
- Author:
Yan HUANG
;
Ruobao LI
- Publication Type:Journal Article
- Keywords:
Rats,Wistar,Tobacco smoke pollution;
Genes,p53;
Ttansforming growth factor beta;
Reverse transcriptase polymerase chain reaction;
Polymorphism,singly-stranded conformational
- From:
Cancer Research and Clinic
2011;23(11):747-751
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the effects of TGF-β1 and p53 on expressions and mutations in smoked lung tissues.Methods Smoked rats model was built.The expressions of TGF-β1 and p53 in lung tissues were observed by immunohistochemistry.The expressions of TGF-β1 mRNA in lung tissues were observed by trans-reverse polymerase chain reaction (RT-PCR).The mutations of p53 in exon 5,6,7-8 were analyzed by polymerase chain reaction single-strandconformational polymorphism (PCR-SSCP).Results The expressions of TGF-β1 in normal lung tissues were faint,and the expressions increased along with the smoking months (P ＜0.05).The positive ratio of protein expression was increased with prolonging smoking time.The mutation of p53 was increased with smoking time.The p53 exons 5,6,7-8 have mutations respectively.The ratio of mutations increased along with smoke months.Conclusion The expressions and mutations of TGF-β1 and p53 increase along with smoked time,which can be regarded as references for lung cancer diagnosis.
