Impact of mutation at V2 of gp120 on V3-specific neutralizing antibodies
10.3760/cma.j.issn.0254-5101.2011.07.009
- VernacularTitle:HIV-1包膜蛋白V2区突变对V3区特异的中和抗体的影响
- Author:
Musang LIU
;
Matsushita SHUZO
;
Shibata JUNJI
;
Weida LIU
- Publication Type:Journal Article
- Keywords:
HIV;
V2 region;
V3 region;
Mutation;
Neutralizing antibody
- From:
Chinese Journal of Microbiology and Immunology
2011;31(7):613-617
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the impact of V2 mutations on neutralizing ability of HIV-1-specific neutralizing antibodies. Methods We tested the influence of L175P mutation to the neutralizing ability of V3-specific antibodies by pseudotype virus and the binding affinity of those V3-spesific antibodies to gpl20 monomer by ELISA. Results We found L175P mutation changed the neutralizing ability of V3-specific antibodies. However, L175P mutation showed no effects on the binding affinity of these antibodies to gpl20 monomer. Conclusion Our results revealed the L175P mutation at V2 loop changed the natural trimmer structure of gp120 and enhanced the neutralizing ability of V3-specific antibodies.
