Occult hepatitis B virus infection in patients with hepatitis B e antibody positive alone
10.3760/cma.j.issn.1000-6680.2012.03.003
- VernacularTitle:单项抗-HBe阳性患者中的隐匿性乙型肝炎病毒感染
- Author:
Xiangyan HUANG
;
Xiangjuan HUANG
;
Qian SHEN
- Publication Type:Journal Article
- Keywords:
Hepatitis B e antigens;
Hepatitis B;
Hepatitis B surface antigens;
Serologic tests;
Mutation;
Gene amplification;
Polymerase chain reaction
- From:
Chinese Journal of Infectious Diseases
2012;30(3):137-140
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo further explore the occurrence of occult hepatitis B virus (HBV)infection m patients with hepatitis B e antibody (anti-Hbe) positive alone and analyze the possible reasons of occult infection.MethodsSera of 61 patients carrying anti Hbe alone (absorbance≤0.1) were collected and real time polymerase chain reaction (PCR) was used to detect HBV DNA level.HBV markers were detected again by Abbott reagent,preS/S amplification products were obtained by PCR,and clonal sequencings were done in HBV DNA positive samples.ResultsTwo samples were HBV DNA positive in 61 samples with anti-Hbe positive alone,with the hepatitis B surface antigen (HBsAg) miss rate of 3.3％.Sequencing disclosed preS deletion mutations,preS2 initiatior codon mutations and co-existence of the different mutant types in one sample with anti-Hbe positive alone by repeated Abbott detection.No preS/S mutations were found in the other sample with HBsAg and hepatitis B core antibody (anti-HBc) weakly positive by repeated Abbott detection except for anti Hbe strongly positive. Conclusions Occult HBV infection and HBsAg serological test failure exist in patients with anti-Hbe positive alone.The failure of HBsAg detection may be due to preS/S gene mutations as well as low level of circulating HBsAg.
