The influence of EGR-1 on NF-κB in HTLV-1 Tax protein positive cells
10.3760/cma.j.issn.0254-5101.2011.06.014
- VernacularTitle:T细胞白血病病毒1型Tax蛋白阳性细胞中早期生长反应基因-1对NF-κB活性的影响
- Author:
Zhiguo NIU
;
Zhihao YU
;
Liyuan CHEN
;
Qingsong HUANG
;
Pan GAO
;
Yuhui HE
;
Hui WANG
- Publication Type:Journal Article
- Keywords:
T lymphocytes;
HTLV-1;
EGR-1;
Tax;
NF-κB
- From:
Chinese Journal of Microbiology and Immunology
2011;31(6):532-536
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the relation of early growth response gene-1(EGR-1) and NF-κB in human T-cell leukemia virus type 1(HTLV-1) Tax protein positive cells. Methods RT-PCR was used to amplify the aimed segments EGR-1 cDNA which was then inserted into an eukaryotic expression plasmid pcDNA3.0 to construct pcDNA3.0-EGR-1. The constructed plasmid was transfected into TaxN and TaxP cells by Tfx-50-mediated transfer method, the expression levels of EGR-1, p65 and Tax mRNA in transfected cells were assay by RT-PCR after 48 h post-transfection, the proteins of EGR-1 and p65 were detected by Western blot after 48 h post-transfection too. The constructed plasmid and pNF-κB-luc reporter gene plasmid was co-transfected into TaxN and TaxP cells by Tfx-50-mediated transfer method, and the activity of luciferase was assay after 48 h post-transfection. Results The results showed that the eukaryotic expression plasmid pcDNA3.0-EGR-1 was successfully constructed. The mRNA and protein expression of EGR-1 could be promoted significantly by Tax. EGR-1 can promote the mRNA and protein expressions of p65 in TaxP cells, the activity of NF-κB was up-regulated by EGR-1 too. Conclusion EGR-1 maybe involve in adult T-cell leukemia(ATL) by increasing the activation of NF-κB.
