Effects of advanced glycation end products on cell viability and level of reactive oxygen species(ROS)in MIN6 cells

VernacularTitle:糖化终末产物对MIN6细胞活力及活性氧(ROS)水平的影响
Author: Shichun DU ; Ning LIN ; Qinmin GE ; Weixia JIAN ; Yan DONG ; Qing SU
Publication Type:Journal Article
Keywords: Advanced glycation end-products; MIN6 cells; Oxidative stress; Reactive oxygen species
From: Chinese Journal of Endocrinology and Metabolism 2011;27(2):152-154
CountryChina
Language:Chinese
Abstract: To explore the effect of advanced glycation end-products(AGEs)on cell viability and level of reactive oxygen species(ROS)in MIN6 cells. After intervention of various concentrations(100,200, and 400 mg/L)of AGEs for some time, cell viability was detected by MTT assay. 2', 7'-dichlorofluorescein diacetate(DCFH-DA)was used as a reactive oxygen species capture agent. The fluorescent intensity of 2', 7'-dichlorofluorescein(DCF), which was the product of cellular oxidation of DCFH-DA, was detected by flow cytometry. The level of ROS and insulin secretion was thus measured. Viability of MIN6 cells was inhibited by AGEs in a dose and time dependent manner(P＜0.05).Intracellular fluorescent intensity of DCF was markedly elevated in the AGEs groups as compared with that in the control group(P＜0.05).Insulin secretion was decreased in the AGEs groups than that in the control group(P＞0.05). The results suggest that AGEs inhibit the viability and induce oxidative stress in MIN6 cells by overproduction of ROS.