Introduction of apoptosis of human hepatoma cells HEPG-2 with liposome-mediated c-myc antisense phosphorothioate oligodeoxynucleotide and 5-FU
10.3760/cma.j.issn.1007-631X.2011.03.026
- VernacularTitle:脂质体介导的c-myc反义硫代寡核苷酸联合5-氟尿嘧啶诱导肝癌细胞HEPG-2的凋亡
- Author:
Tiankang GUO
;
Hui CAI
;
Yanqing SUN
;
Zhenjun LI
;
Li WEI
- Publication Type:Journal Article
- Keywords:
Carcinoma,hepatocellular;
Apoptosis;
Gene,c-myc
- From:
Chinese Journal of General Surgery
2011;26(3):246-250
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTostudytheinhibitioneffectof c-mycASODN(antisense oligodeoxynucleotide) and 5-FU (5-fluorouracil) on the expression of c-myc gene and the proliferation of human hepatomacellsHEPG-2. MethodsAfter treatedbyliposomemediatedc-mycantisense phosphorothioate oligodeoxynucleotide (APSODN) and 5-FU, the growth inhibition rate was detected by MTT assay, the expression of c-myc mRNA was detected by RT-PCR and immunohistocehemical methods HEPG-2cells. The cell cycle was analyzed by flow cytometric analysis. The morphological changes were observed by fluorescence staining and cellular genome electrophoresis. ResultsAfter sealing c-myc gene with ASODN,the growth of cells was repressed and the effect was time-dependent and dose-dependent ( P = 0. 02 ). The ability of proliferation decreased, the expression of c-myc gene was inhibited on transcription and translation levels; 5-FU can induce apoptosis of hepatoma cells HEPG-2 dramatically with the dose of 10 μ mol/L, when treated by both c-myc ASODN and 5-FU, HEPG-2 cells was induced apoptosis in a cooperative style ( P =0. 01 ).ConclusionsThe liposome mediated c-myc (APSODN) and 5-FU can inhibit the proliferation of HEPG-2 cells by inhibiting the expression of c-myc gene and can induce apoptosis of hepatoma cells HEPG-2 in a cooperative style. c-myc ( APSODN ) can increase the sensitivity of 5-FU to hepatoma cells and decrease the effective concentration of 5-FU.
