Quantitative detection of K-ras gene mutation in samples from endoscopic ultrasound-guided fine-needle aspiration for pancreatic cancer

VernacularTitle:胰腺超声内镜细针穿刺活检物中K-ras基因定量检测对胰腺癌诊断价值的研究
Author: Xiaowei WANG ; Jun GAO ; Junjun GU ; Hua LU ; Zhendong JIN ; Zhaoshen LI ; Yanfang GONG ; Jing JIN
Publication Type:Journal Article
Keywords: Pancreatic cancer; Endoscopic ultrasonography; Puncture biopsy; Genes,ras
From: Chinese Journal of Digestive Endoscopy 2011;28(3):126-130
CountryChina
Language:Chinese
Abstract: Objective To investigate the diagnostic value of a quantitative detection of K-ras mutation in samples from endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA)of pancreatic cancer.Methods Samples taken by EUS-FNA from 53 pancreatic occupying lesions were collected, and the copies of wild-type and mutated K-ras gene was measured by PNA-clamping real-time quantitative PCR. The results were analyzed with refer to cytological findings to evaluate its clinical values. Results According to cytological finding, a total of 37 cases were diagnosed as pancreatic cancer, and 16 were non-malignant lesions. Kras mutation was detected in 83.8% of cancer cases, and 18. 8% of non-cancer cases, which was significantly different ( P ＜0. 05 ). Sensitivities of cytology and K-ras examination were 59. 5% and 83.8%, respectively, while that of combination of cytology and K-ras examination was 89. 2%. Conclusion Quantitative analysis of the mutant K-ras gene in samples taken by EUS-FNA is a useful tool for diagnosing the pancreatic carcinoma.