Expression of L - selectin in experimental allergic encephalomyelitis of Wistar rats and its clinical significance
10.3760/cma.j.issn.1008-6706.2011.12.002
- VernacularTitle:实验性变态反应性脑脊髓炎大鼠L-选择素的表达及意义
- Author:
Hezhong ZHENG
;
Kewen XU
;
Wei DONG
;
Yulan WANG
;
Qi NI
;
Jing WENG
;
Hong YI
- Publication Type:Journal Article
- Keywords:
Experimental allergic;
Encephalomyelitis;
L-selectin
- From:
Chinese Journal of Primary Medicine and Pharmacy
2011;18(12):1587-1589
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of L-selectin in experimental allergic encephalomyelitis (EAE) of Wistar rats.Methods The rats were randomly divided into four groups;the normal group,the CFA group, the LMS group and the model group;The animal models were established in rats by immunization with myelin basic protein of spinal cord of guinea pig and complete Freund's adjuvant(CFA).The symptom of EAE was observed; pathological feature and myelin of brain and spinal were detected with HE stain and Loyez's stain respectively.The number of positive vessels of L-selectin expression was detected by immunohistochemistry.Results 100% experimental Wistar rats treated with MBP and levamisole developed EAE,but none of the other groups.The number of positive vessels of L-selectin expression was (31.86 ± 1.39) in model group, obviously higher than that of in the normal group (1.38 ±0.18) ,the CFA group( 1.50 ±0.27) and the LMS group(7.25 ±0.59) (all P <0.05) ;The inflammation cells were found around vessels and demyelination were seen in white matter of brain and spinal cords.Conclusion The expression of L-selectin should play an important role in EAE.
