Construction of expression plasmid in human WAF1 gene
- VernacularTitle:人WAF1基因表达质粒的构建
- Author:
Zhifu HOU
;
Weizhong WANG
;
Hua WANG
;
Xiaodong WU
;
Lisha PU
;
Liying WANG
- Publication Type:Journal Article
- Keywords:
WAF1;
gene cloning;
direct sequancing
- From:
Journal of Jilin University(Medicine Edition)
2000;26(6):559-561
- CountryChina
- Language:Chinese
-
Abstract:
Objective :Humna WAF1 gene was cloned,and WAF1-pcDNA3 recombinant plasmid was con-structed. Methods :Human WAF1 gene was amplified by RT-PCR from Hela cell,then cloned into pcDNA3vector. Results :Human WAF1-pcDNA3 recombinant plasmid was constructed,and DNA direct sequencingindicated that was correct. Conclusion:Our study laid the foundation for studying WAF1 gene expressionand it＇s biological effects.
