Study on metastasis-associated gene in carcinoma by cDNA microarray
- VernacularTitle:肿瘤转移相关基因的表达谱研究
- Author:
Juxiang CHEN
;
Rong TANG
;
Kang YING
;
Gang JIN
;
Yao LI
;
Quansheng YANG
;
Zhiren FU
;
Jingping FAN
;
Ju MEI
;
Yi XIE
;
Yicheng LU
;
Yumin MAO
- Publication Type:Journal Article
- Keywords:
DNA microarray;
carcinoma;
gene,metastasis-asso ciated
- From:
Academic Journal of Second Military Medical University
2000;21(9):806-811
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the molecular pat hophysiology of hepatocellular carcinoma and pancreatic cancer.Methods: We studied novel gene expression by cDNA microarray method. The PCR pro ducts of 4 096 genes and 12 800 gene were spotted onto a kind of chemical-mater ial-coated-glass slide in array. Both the mRNAs from 5 cases of hepatocellular carcinoma and 3 cases of pancreatic cancer were reversely transcribed to cDNAs with the incorporation of fluorescent-labeled dUTP to prepare the hybridization probes. After hybridization, BioDoor4096 and BioDoor12800 cDNA microarray were scanned for the fluorescent intensity. Tumor invasion-related gene expression w as screened through the analysis of difference in gene expression profile.Results:Among 4 096 and 12 800 target genes, there were 15 genes who se expression level differed from normal and carcinoma tissues. Therefore, they might be associated with metastasis.Conclusion:Further analysis of these differentially expressed metastasis-associated genes will be helpful for understanding the molecular mechanism of malignant carcinoma.
