Study of Location in Tumor Bearing Nude Mice of Monoclonal Antibody to Human Esophageal Carcinoma
- VernacularTitle:抗人食管癌单克隆抗体在荷瘤裸鼠体内的定位
- Author:
Changzheng LIU
;
Junfang HE
;
Changsheng LIANG
;
Peng LIN
;
Jinhui GAO
;
Lehe LIU
;
Zhongying SHEN
- Publication Type:Journal Article
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2001;22(1):19-21
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To study the location effect of the Mc Ab G9 to human esophageal carcinoma in tumor bearing nude mice. 【Methods】 125 I-G9 was prepared by Chloramine-T method. The distribution of 125 I-G9 was detected at different time (24, 48, and 72 h) after peritoneal injecti on. The percentage of the injected dose per gram of tissue(%ID/g) and the ratio of Tumor/non-Tumor were calculated. 【Results】 The distribution of 125 I -G9 in tumor at the third day was showed by autoradiography obviously higher th an in other organ/tissue (except blood) and the highest is at the 48 h. The T/NT values are 2-7. The autoradiography indicated that radioactivity concentrated in tumor tissue. The concentration in tumor edge is more obvious than in the ce nter. 【Conclusion】 125 I-G9 has a considerable targeting activity and can well locate in esophageal carcinoma tissue in tumor bearing nude mice.
