Chemosensitivity Test of Human Head and Neck Tumors Cell Line with MTT Assay
- VernacularTitle:MTT分析法在头颈肿瘤体外药敏试验中的应用
- Author:
Deping ZHAO
;
Chun XIE
;
Liu LIU
;
Xuemei QI
;
Xiling CHEN
;
Xin LU
- Publication Type:Journal Article
- From:
Journal of Kunming Medical University
2001;22(1):34-38
- CountryChina
- Language:Chinese
-
Abstract:
In vitro cell culture of head and neck tumors and chemosensitivity test to select sensitivity antitumor bogs for individual chemotherapy. Methods:The chemosensitivity of 32 fresh specimens of human head and neck tumors were tested with MTT assay and eight atitumor drugs． Results:The sensitivity was 84.4% for 5-Fu, 2l.9% for VCR, 62.5% for MTX, 8l.3% for BLM, 8l.3% for DDP, 40.6% for ADM, 65.6% for PYM and 3.l% for CTX． The results of chemotherapy with sensitivity drugs of 32 patients were encouraged． There were no serious side-effects in the chemotherapy and no recurrence and metastasis after the treatment． 32 patients were still alive． Conclusion: Chemosensitivity test of tumor cell lines with MTT assay was accuracy， fast，simple，ecoromical，effective method and was valuable for chemotherapy．
