Experimental study on arsenic trioxide-induced apoptosis of human pan creatic cancer cell line
- VernacularTitle:三氧化二砷诱导人胰腺癌细胞凋亡的实验研究
- Author:
Yong GAO
;
Jiejun WANG
;
Qing XU
;
Jing GUO
;
Shaochang JIA
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2001;22(1):43-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of arsenic trioxide (As2O3) on human pancreatic cancer cell proliferation and apoptosis (mainly early stage) in vitro. Methods: SW1990 cells line were trea ted with As2O3 at different concentration. Cell proliferation was evaluated by MTT and apoptosis by Annexin-Ⅴ-fluostaining, electron-microscopy, flow cytometry and immunocytochemical staining of Bcl-2 and Bax. Results: As2O3 and cisplatin had the same cytotoxity on SW1990. The cytotoxic effe ct on tumor cell was produced by induction of apoptosis. Twelve hours after cult ure with 10 μg/ml As2O3, much more SW1990 cells went into apoptosis than t he control. The apoptosis rate reached 24% after 48 h with the similar concentra tion of As2O3. Immunohistochemical study revealed that the expression of Bcl -2 was decreased after treated with As2O3. Conclusion: As 2O3 can depress the proliferation of SW1990 in vitro, mainly through the i nduction of apoptosis, and it is a potential agent for pancreatic cancer chemoth erapy.
