Effect of angiotensin II on the three transcription factors in endothelial cells
- VernacularTitle:血管紧张素II对血管内皮细胞三种转录因子作用的影响
- Author:
Hua WANG
;
Qin SI
;
Xiaoming WANG
;
Hengyi GUO
;
Sumin LI
;
Qixia WU
- Publication Type:Journal Article
- From:
Chinese Journal of Pathophysiology
2001;17(3):196-199
- CountryChina
- Language:Chinese
-
Abstract:
AIM and METHODS: The effects of angiotensin II(AngII) on the activation of three transcription factors were investigated by using EMSA and western-blot methods in endothelial cells respectively. RESULTS: The EMSA results showed that AngII stimulation could increase NF-κB, SP-1 and AP-1 activation in ECV304, which suggested that activity changes in these three transcription factors could partly contribute to the dysfunction of endothelial cells.The binding affinity of NF-κB, SP-1 and AP-1 with corresponding oligonucleotides in AngII-treated ECV-304 were respectively 10.98,3.89,1.33 times as large as in control. The nuclear appearance of AngII-activated NF-κB was examined by western-blot, which corroborates our results from EMSA analysis. While as the protein appearance of AP-1 and SP-1 in nucleus, were little higher than the control group. The result of western-blot suggested that AngII-induced EC activation of these three transcription factors maybe mainly due to the enhanced binding ability to its corresponding cis-acting factors. CONCLUSION: NF-κB, a ubiquitously exposed nuclear transcription factor, is involved, together with SP-1,AP-1, in the regulation of endothelial cell dysfunction related to cardiovascular diseases such as atherosclerosis and hypertension.
