INTRATHECAL ADMINISTRATION OF PRODYNORPHIN ANTISENSE OLIGODEOXYNUCLEOTIDE SUPPRESSES BOTH PRODUCTION OF DYNORPHIN A IN DORSAL HORN AND BEHAVIORAL NOCICEPTIVE RESPONSES INDUCED BY FORMALIN TEST
- VernacularTitle:大鼠鞘内注入前强啡肽原的反义寡聚核苷酸减弱福尔马林引起的脊髓背角中强啡肽A的合成和行为痛反应:细胞免疫化学和行为学联合观察
- Author:
Wenxiu QI
;
Yu ZHANG
;
Jinshun QI
;
Jiantian QIAO
- Publication Type:Journal Article
- Keywords:
preprodynorphin;
antisense oligodeoxynucleotide;
dynorphin A;
Fos protein;
behavioral nociceptive response;
spinal dorsal horn;
rat
- From:
Chinese Journal of Neuroanatomy
2003;19(4):351-358
- CountryChina
- Language:Chinese
-
Abstract:
By using intrathecal administration of antisense oligodeoxynucleotide (AS-ODN) against preprodynorphin mRNA in rats, we observed that this treatment could block both the formalin-induced behavioral nociceptive responses and the increased expression of dynorphin A (1-8) in the dorsal horn, with the increased expression of c-Fos protein being unchanged. For we have reported that intrathecal administration of AS-ODN against c-fos mRNA blocks the nociceptive responses and both the increased Fos protein and dynorphin A (1-8), the results of the present study suggest that: (1) Nociceptively induced spinal expression of dyorphin and Fos protein is involved in the transmission of nociceptive information at the spinal level and the expression of Fos protein is the up-stream event. (2) dynorphin may act as a pronociceptive, not an antinociceptive, factor in the modulation of the spinal hyperalgesic state.
