Growth-inhibitory effects of selective cyclooxygenase-2 inhibitor on colon cancer cells and its possible mechanisms
- VernacularTitle:选择性环氧合酶-2抑制剂对结肠癌细胞的生长抑制作用及机制探讨
- Author:
Xiaoqing JIA
;
Lihui HAN
;
Ning ZHONG
;
Fanli MENG
;
Ming YAN
;
Wenjie LI
;
Yanqing LI
;
Shangzhong ZHANG
- Publication Type:Journal Article
- Keywords:
Cyclooxygenase inhibitors;
Apoptosis;
Colonic neoplasms
- From:
Chinese Journal of Pathophysiology
2005;21(5):985-989
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To evaluate the growth-inhibitory effects of NS-398, a selective cyclooxygenase-2 inhibitor, in human colon cancer HT-29 cells and its possible mechanisms. METHODS: MTT assay was applied to detect the cell proliferation. Flow cytometry was performed to detect apoptosis rate and cell cycle. RT-PCR was used to detect the expression of bcl-2 mRNA and bax mRNA. Alteration of cytoskeleton component F-actin was observed by confocal laser scanning microscope. RESULTS: NS-398 could inhibit growth of HT-29 cells in dose-and time-dependent manners. Flow cytometry revealed that NS-398 could induce apoptosis and cause G0/G1 arrest of HT-29 cells in a dose-dependent manner. After 72 h incubation with NS-398 at different concentrations, the expression level of bcl-2 mRNA was lowered and the ratio of bcl-2 to bax was decreased in HT-29 cells. F-actin was mainly distributed around nuclei forming annular structure in HT-29 cells. After exposure to NS-398, the annular structure around nuclei disappeared and fluorescence intensity of F-actin decreased obviously. CONCLUSION: NS-398 can inhibit the growth effectively and induce apoptosis in HT-29 cells in vitro, which is associated with the down-regulation of bcl-2 to bax ratio and the disruption of cytoskeleton.
