Ginsenoside-Ro enhances cell proliferation and modulates Th1/Th2 cytokines production in murine splenocytes
- VernacularTitle:人参皂苷-Ro促进小鼠脾细胞增殖及调节小鼠脾细胞Th1/Th2细胞因子的产生
- Author:
Junli YU
;
Deqiang DOU
;
Xiaohong CHEN
;
Hongzhen YANG
;
Xiaoyan HU
;
Guifang CHENG
- Publication Type:Journal Article
- Keywords:
ginsenoside-Ro;
IL-2;
IL-4;
IFN-γ;
splenocytes
- From:
Acta Pharmaceutica Sinica
2005;40(4):332-336
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effects of ginsenoside-Ro on cell proliferation and cytokine production in murine splenocytes. Methods The effect of ginsenoside-Ro on murine splenocytes proliferation was studied using [3 H] thymidine incorporation assay. Effects of ginsenoside-Ro on the production of cytokines interleukin-2 (IL-2), interferon-γ (IFN-γ) and interleukin-4 (IL-4) from murine splenocytes were detected by ELISA method. Effects of ginsenoside-Ro on mRNA level of Th1 cytokine IFN-γ and Th2cytokine IL-4 were evaluated by reverse transcription polymerase chain reaction (RT-PCR) analysis.Results Ginsenoside-Ro showed no mitogenic effect on unstimulated murine splenocytes. It enhanced the proliferation of Con A-induced murine splenocytes and the production of IL-2 at concentrations of 1 - 10decreased the production and expression of Th1 cytokine IFN-γ in Con A-induced murine splenocytes at regulating the production and expression of Th1/Th2 cytokines in murine splenocytes.
