CHANGE OF COX-1/2 EXPRESSION IN BRAIN AFTER SPARED NERVE INJURY-INDUCED NEUROPATHIC PAIN AND ANALGESIC EFFECTS OF COX INHIBITORS WITH DIFFERENT SELECTIVITY
- VernacularTitle:神经病理性痛后全脑环氧合酶表达的变化及不同选择性环氧合酶抑制剂镇痛效应的比较
- Author:
Zhihong LU
;
Xiaoyun XIONG
;
Guocheng LIN
;
Jingru MENG
;
Qibing MEI
- Publication Type:Journal Article
- Keywords:
neuropathic pain;
cyclooxygenase ( COX);
COX selective inhibitors;
radioimmunoassay;
RT-PCR;
Western blotting;
mice
- From:
Chinese Journal of Neuroanatomy
2006;22(1):27-32
- CountryChina
- Language:Chinese
-
Abstract:
To observe the expression of cyclooxygenase (COX)-1 and COX-2 in brain after spared nerve injury (SNI) and compare the analgesic effects of COX inhibitors with different selectivity. Radioimmunoassay, RT-PCR and Western blotting techniques were used to evaluate the change of brain COX expression at different time points( before SNI, 1 h, 12 h, 1 d, 3 d, 7 d, 14 d, 30 d and 60 d after SNI); By exploring hot plate test, we observed the reacting time of animals after injection of saline, NS-398, SC-560 and indomethacin at different time points. The results showed that: ( 1 ) The expression of brain COX-1 didn't increase significantly until 14 d after SNI, while that of COX-2 increased significantly and rapidly after SNI and reached peak at the time point of 1 d ( all P ＜0.05 ); (2) NS-398 showed significant analgesic effect on neuropathic pain after SNI at the early phase ( P ＜ 0.05 ), but didn't persist for over 30 d; ( 3 ) Indomethacin and SC-560 didn't show significant analgesic effects until 14 d. These results suggest that brain COX-1 is involved in the late phase of neuropathic pain and may play a role in the persistence of pain, while brain COX-2 is involved in the early phase of neuropathic pain and may play a role in the pain origination.
