Calcium caused calcium release causes a vascular smooth muscle cell line A10 cells apoptosis
- VernacularTitle:钙诱导的钙释放引起一株血管平滑肌细胞株凋亡
- Author:
Yulin WU
;
Bingliang MA
;
Xiaoming DAI
;
Linlin CHEN
- Publication Type:Journal Article
- Keywords:
vascular smooth muscle cell;
apoptosis;
calcium release;
ryanodine;
mitochondria
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2006;11(7):789-796
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the apoptosis of a vascular smooth muscle cell line A10 caused by mild K+ depolarization. METHODS: Apoptosis was evaluated by nuclear staining, DNA fragmentation gel electrophoresis and propidium iodide-stained flow cytometry. Mitochondrial transmembrane potential (Δψm) was measured by flow cytometry. RESULTS: K+ depolarization caused dose correlated A10 cells apoptosis; nifedipine, BAPTA/AM, ryanodine inhibited the cytotoxic effect of K+ completely.The combination use of nifedipine and cyclosporin A made it clear that mitochondria was involved in the apoptosis of A10 cells,and Δψm measurement further confirmed this speculation; A10 apoptosis caused by K+ depolarization was not influenced by heparin or Zn2+,a effective capacitative calcium entry(CCE) blocker. CONCLUSION: Ca2+ entry through voltage-dependent ca channels increases intracytoplasm Ca2+, then triggers further Ca2+ release from endoplasmic reticulum via ryanodine receptor, and the microdomains of elevated intracytoplasm Ca2+ are sensed by adjacent mitochondria, which ultimately lead to cell apoptosis.
