Changes in Global Gene Expression Induced by NOR1 Over-expression in HepG2 Cells
- VernacularTitle:NOR1基因转染对肝癌细胞HepG2基因表达谱的影响
- Author:
Dengqing LI
;
Hua TANG
;
Rong GUI
;
Xinmin NIE
- Publication Type:Journal Article
- Keywords:
NOR1;
hepatoma;
cDNA microarray;
quantitative real-time PCR.
- From:
Progress in Biochemistry and Biophysics
2008;35(4):457-464
- CountryChina
- Language:Chinese
-
Abstract:
Previous work from this laboratory has cloned a novel gene NOR1 and showed its extensive expression in normal tissues and down-regulation in carcinomas. To further investigate its downstream target genes and better understand its function, NOR1 was over-expressed in HepG2 hepatoma cells and global changes in gene expressions from a stable line were identified by cDNA microarrays. The results discovered 59 genes up-regulated in these cells compared with the original cells, including Grb2, HBP17,TNFRSF11B genes that have been implicated in tumorigenesis and cancer development. In addition, 103 down-regalated genes were also identified, including genes encoding Bik, MAP2K6 and ZFP95 proteins. The expression patterns of certain genes identified by microarrays were validated by quantitative real-time PCR and the results showed that expression difference were statistically significant (P< 0.05). These data suggest that NOR1 may influence the biology and cancerous behaviors of HepG2 cells by regulating expression of a set of genes involved in signal traasduction, cell cycle regulation, transcription and Wanslation controls.
