Inhibition of Newcastle disease virus (NDV) replication by RNA interference targeting phosphoprotein (P) gene in CEF
- VernacularTitle:利用RNAi技术靶向新城疫病毒P基因抑制其在鸡胚成纤维细胞上的复制
- Author:
Lianzhi MU
;
Zhuang DING
;
Yanlong CONG
;
Renfu YIN
;
Mei LIU
;
Changqing WANG
;
Shaoli LI
;
Mimi QIU
- Publication Type:Journal Article
- Keywords:
Newcastle disease virus;
RNA interference;
CEF;
inhibition
- From:
Chinese Journal of Veterinary Science
2009;29(7):841-844
- CountryChina
- Language:Chinese
-
Abstract:
A plasmids of continuous expressing shRNAs targeting the NDV NA-1 Phosphoprotein (P) gene was designed.Virus titration,Real Time RT-PCR,CPE indicated that P-specific siRNA could inhibit virus replication at 36 h post-virus infection.In future studies,a combination of siRNAs targeting the NP and L gene may be used as a tool to study NDV replication and antiviral therapy.
