Isolation and biological properties of avian infectious bronchitis virus isolated from Shanxi province
- VernacularTitle:鸡传染性支气管炎病毒地方流行株的分离与鉴定
- Author:
Fang YAN
;
Wenbin YUE
;
Juan LIU
;
Xuying LI
;
Yujun ZHAO
;
Wenhui JI
;
Fengbo LIU
;
Qian WU
;
Jiayan REN
;
Lizhen HUA
- Publication Type:Journal Article
- Keywords:
infectious bronchitis virus;
isolation;
characterizing
- From:
Chinese Journal of Veterinary Science
2009;29(7):845-848
- CountryChina
- Language:Chinese
-
Abstract:
Five field strains of infectious bronchitis virus (IBV) were isolated from suspected flocks from different time and different regions of Shanxi province,respectively,and characterized by a series of systematic identification assays,such as morphological observation by electron-microscope,interfering with the propagation of NDV,virus pathological role to chicken embryo,virus pathological role to SPF chickens,hemagglutination activity,physiscochemical,and reverse transcription polymerase chain reaction(RT-PCR).The results showed:The typical coronavirus which the spherical virions 60-120 nm in diameter and surface covered with spike like corona were observed under electron-microscope)The propagation of NDV strain was seriously interfered by the 5 isolates respectively;The embryonated chicken egg passages of the 5 isolates could dwarf with chicken embryos;The five isolates had no hemagglutination activity,but after treatment with 1% trypsin,it can agglutinate chicken red blood cell.The strains are sensitive to chloroform and ethyl ether.The SPF chickens which inoculated with the 5 isolates showed clinical sign and result in respiratory and kidney diseases,flower-steak,and swollen with severe urate deposition.The specific fragments of N gene of the 5 isolates were amplified by RT-PCR,respectiveiy.On the basis of all above mentioned results,the 5 isolates were classified as IBV.The study got a good preparedness for further study on molecular epidemioogy of the 5 IBV isolates.
