Expressions of TGFβ1 and TGFβR1 in primary and metastastic pancreatic cancer cell lines
- VernacularTitle:原发性及转移性胰腺癌细胞中TGFβ1和TGFβR1的表达
- Author:
Jian CHEN
;
Liang ZHONG
;
Dongni QIU
;
Dayu SUN
- Publication Type:Journal Article
- Keywords:
pancreatic carcinoma;
transforming growth factor beta;
receptor
- From:
Fudan University Journal of Medical Sciences
2009;36(4):431-434
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the different expressions of transforming growth factor beta1 (TGFβ1) and its type one receptor(TGFβR1) between primary pancreatic cancer cell line AsPC-1 and metastatic pancreatic cancer cell line BxPC-3. Methods The mRNA expressions of TGFβ1 and TGFβR1 in AsPC-1 and BxPC-3 pancreatic carcer cell lines were quantatitived by real-time RT-PCR. The protein levels of TGFβ1 and TGFβR1 in these two cell lines were measured by Western blot assay. Results Compared with the primary pancreatic cancer cell AsPC-1, the mRNA and protein expressions of TGFβ1 and TGFβR1 were much higher in BxPC-3 pancreatic cancer cells(P<0.05). Conclusions Upregulations of TGFβ1 and TGFβR1 might be a pivotal incidence in the procedure of malignant progressing and metastasis in pancreatic cancer cells.
