A proteomic study on the insulin resistant HepG_2 cell treated by Ecdysterone
- VernacularTitle:蜕皮甾酮对胰岛素抵抗HepG_2细胞的蛋白质组学研究
- Author:
Min SONG
;
Yaojun LI
;
Guoqi LAI
;
Zongyin QIU
- Publication Type:Journal Article
- Keywords:
Ecdysterone;
insulin resistant;
proteome;
2-DE;
HepG_2 cell;
mass spectrometry analysis
- From:
Chinese Pharmacological Bulletin
2009;25(12):1640-1644
- CountryChina
- Language:Chinese
-
Abstract:
Aim To screen the express-altered proteins before or after effect of Ecdysterone on HepG_2 cell model of insulin resistance by the strategy of comparative proteomics, which may approach new proves exploring the target of sensitizer.Methods HepG_2 cells were incubated with 5×10~(-7) mol·L~(-1) insulin for 16 h, and glucose consumption was determined. After treatment, the insulin resistant cells were incubated with 10~(-5) mol·L~(-1) Ecdysterone for 24 h.Then glucose consumption contents were determined. The proteins of two groups before and after treatment with Ecdysterone were extracted by lysis buffers. The express-altered proteins were screened by 2-DE technique.Some of them were analyzed by MALDI-TOF-MS mass spectrometry and MS-Fit database.Results 53 express-altered protein spots of insulin resistant HepG_2 cells before and after treated by Ecdysterone were screened by 2-DE technique,in which 35 ones were up-regulated and the others down-regulated, 6 spots of which were analyzed by MALDI-TOF-MS mass spectrometry and MS-Fit database.Conclusion The target of Ecdysterone as a sensitizer involves many proteins and kinases which correlate insulin resistant. These results lay a foundation for further studies on the function of these target proteins.
