Effects of peptide fragments from the proadrenomedullin on cAMP——"The intramolecular regulation" of signal transduction pathway in cells
- VernacularTitle:肾上腺髓质素前体肽来源的肽段对cAMP的影响——在细胞内信号转导途径的"分子内调控"现象
- Author:
Xianhong DONG
;
Ruanling HOU
;
Jianzhi GAO
;
Chaoshu TANG
- Publication Type:Journal Article
- Keywords:
proadrenomedullin;
adrenomedullin;
pro-adrenomedullin N-terminal 20 peptide;
adrenotension;
cAMP;
aorta
- From:
Chinese Pharmacological Bulletin
2009;25(12):1575-1578
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of Peptide fragments that originate from the same proadrenomedullin on cAMP in rat aorta.Methods Isolated aortic tissues were exposed to ADM,PAMP and ADT for 2 h.The content of cAMP in the incubated media was assayed by radioimmunoassay.Results ① The content of cAMP in the aortic tissues that were exposed to ADM (10~(-7) and 10~(-8) mol·L~(-1)) was significantly increased. The content of cAMP in the aortic tissues stimulated by PAMP (10~(-8) mol·L~(-1))or ADT (10~(-8) mol·L~(-1))alone was significantly increased, compared with the control.② The content of cAMP was not in-creased when the tissues were treated with ADM (10~(-8) mol·L~(-1)) and PAMP (10~(-8) mol·L~(-1)) or ADT (10~(-8) mol·L~(-1)) in combination, compared with that after the treatment with ADM(10~(-8) mol·L~(-1))alone, but was significantly decreased than that in PAMP(10~(-8) mol·L~(-1)) or ADT(10~(-8)mol·L~(-1)) groups. After incubation with ADM,PAMP and ADT at the same dose (10~(-8) mol·L~(-1)), the content of cAMP did not change as compared with that of the ADM group (10~(-8) mol·L~(-1),P>0.05), but was greatly reduced, compared with that of the PAMP or ADT groups(10~(-8) mol·L~(-1),P<0.01).Conclusion ADM, PAMP and ADT which originate from the proadrenomedullin have an antagonism on cAMP production in rat aorta.
