Urotensin Ⅱ aggravated β-glycerophosphate-stimulated calcification in cultured rat vascular smooth muscle cells
- VernacularTitle:尾加压素Ⅱ加重β-甘油磷酸诱导的大鼠血管平滑肌细胞钙化
- Author:
Baohong ZHANG
;
Xiaobo CHEN
;
Erzhen LI
;
Tianyou WANG
;
Chaoshu TANG
;
Junbao DU
- Publication Type:Journal Article
- Keywords:
calcification;
urotensin Ⅱ;
vascular smooth muscle cells;
cell phenotype;
rat
- From:
Chinese Pharmacological Bulletin
2009;25(12):1567-1570
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of urotensin Ⅱ on vascular calcification.Methods Calcified VSMCs of rat in vitro were induced by β-glycerophosphate.Cellular calcium content,ALP activities,~(45)Ca accumulation and osteocalcin content were measured.Results Compared with those of control group,calcium content,ALP activities,~(45)Ca uptake and osteocalcin in calcified VSMCs increased greatly(P<0.01).Calcium content,ALP activities,~(45)Ca uptake and osteocalcin of calcified VSMCs stimulated by urotensin Ⅱ (10~(-10)、10~(-9) and 10~(-8) mol·L~(-1))were greatly increased in a concentration-dependent manner as compared with those of calcified group(P<0.01).Conclusion UrotensinⅡ aggravates the calcification of VSMCs induced by β-glycerophosphate.
