The Unscheduled Expression of Cyclin B1/CDK1 Contributes to the Transition of Cell Cycle of Lymphocytes in vivo from G_0 to G_1 Phase

VernacularTitle:异时相Cyclin B1/CDK1表达有助于正常在体细胞进入细胞分裂周期
Author: Yonghong ZHANG ; Dongdong YU ; Leya HE ; Xin WEI ; Yan LENG ; Deding TAO ; Junbo HU ; Jianping GONG
Publication Type:Journal Article
Keywords: unscheduled expression; cell division cycle; peripheral blood lymphocyte
From: Acta Medicinae Universitatis Scientiae et Technologiae Huazhong 2009;38(6):793-795
CountryChina
Language:Chinese
Abstract: Objective To study the mechanism of unscheduled Cyclin B1 expression at G_1 phase which is usually at G_2/M phase.Methods Human peripheral blood lymphocytes(PBL) from healthy volunteers were firstly activated by PHA and then went into cell cycle.The cells were collected at 0,36,48 and 60 h after activation and divided into two parts:one for Cyclins/DNA muhiparameter assay,and another for Post-sorting Western blot.Results After activation by PHA,Cyclin B1 and CDK1 of lymphocytes were expressed at G_1 phase.Conclusion Unscheduled Cyclin B1/CDKl probably contributes to lymphocytes in vivo into cell cycle.