The growth-inhibition of osteosarcoma xenografts by adenovirus-mediated ING4 gene in nude mice
- VernacularTitle:腺病毒介导的ING4对裸鼠人骨肉瘤移植瘤的生长抑制作用
- Author:
Yali HAN
;
Jingcheng MIAO
;
Weihua SHENG
;
Yingying JING
;
Yunbo SHAN
;
Tielian LIU
;
Zhengyi LI
;
Wanrong BAO
;
Jicheng YANG
- Publication Type:Journal Article
- Keywords:
Ad-ING4;
Adenoviral vector;
MG-63 osteosarcoma;
Growth-suppression
- From:
Chinese Journal of Immunology
2009;25(12):1070-1074
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the inhibitory effect and anti-cancer mechanisms of adenovirus-mediated ING4 gene on the MG-63 osteosarcoma xenografts in nude mice.Methods:Ad-ING4 was transfected into QBI-293 cells and harvested.15 nude mice of the subcutaneous tumor models were established with MG-63 osteosarcoma cells and were randomly divided into PBS,Ad-GFP and Ad-ING4 groups.Then PBS(100 μl),Ad-GFP(100 μl,10~9pfu/ml) and Ad-ING4 (100 μl,10~9pfu/ml) for each one were given respectively QOD for 5 times,with intratumor injections.Tumor volume changes were monitored;and the 15 mice were sacrificed 2 weeks after treatment,the tumors were removed,weighed and ratios of tumor-suppression were calculated.The morphological changes of apoptotic tumor cells were observed under microscope.Bcl-2,Bax,Caspase-3,VEGF,CD34 expression was tested by immumohistochemistry.Results:High titer(10~9pfu/ml)adenoviral vector of ING4 gene were obtained.In nude mice bearing MG-63 osteosarcoma xenografts,the growth of MG-63 tumors treated by intratumoral injecting of Ad-ING4 was significantly suppressed,compared with PBS group and Ad-GFP group.The ratios of tumor weight-suppression of Ad-ING4 group was 59.3%(P＜0.05).Immumohistochemistry displayed that the expression of Bax,Caspase-3 was up-regulated and the expression of Bcl-2,VEGF,CD34 was down-regulated by Ad-ING4.Conclusion:Ad-ING4 can inhibit the growth of MG-63 osteosarcoma xenografts in nude mice,which may be via activating the apoptosis pathway and inhibiting tumor angiogenesis.
