Progeny of 2-cell embryo blastomeres distribute in mouse blastocyst randomly
10.3969/j.issn.0529-1356.2010.01.024
- VernacularTitle:小鼠2-细胞胚胎卵裂球后代在囊胚中随机分布
- Author:
Zhongwei WANG
;
Shunli YUE
;
Jiawei ZHU
;
Xiaozhang CAO
;
Qi SUN
;
Yuan WANG
;
Jiabo ZHOU
- Publication Type:Journal Article
- Keywords:
Embryo polarization;
Em-Ab axes;
Blastomere;
Microinjection;
Mouse
- From:
Acta Anatomica Sinica
2010;41(1):124-127
- CountryChina
- Language:Chinese
-
Abstract:
Objective Kunming strain(KM) mice were used as animal models. Nontoxic dextran conjugated with tetramethylrhodamine(TMR)and fluorecein isothiocyante(FITC)was microinjected to two of the 2-cell blastomere as molecular probe to trace the development fate of the blastomere ,in order to figure out the mechanisms of the formation of Em-Ab axis. Methods FITC- dextran was injected to zygote in order to make sure if it is noxious. Two blastomeres of 2-cell embryo were injected FITC- dextran and TMR- dextran respectively. Results When labeled embryo develeped to blastocyst, distribution of progeny of 2-cell embryo blastomeres can be detected.Conclusion The cells of blastomere randomly distributed either embryonic parts or extraembryonic parts of blastocyst.
