Distribution of α-synuclein truncations in cells
- VernacularTitle:α-synuclein C端截短体的胞内分布
- Author:
Kaili MA
;
Yuhe YUAN
;
Jinfeng HU
;
Jiandong SUN
;
Yan LIU
;
Boyu LI
;
Naihong CHEN
- Publication Type:Journal Article
- Keywords:
α-synuclein;
truncations;
nuclei-distribution;
MN9D cells;
PC12 cells;
SH-SY5Y cells
- From:
Chinese Pharmacological Bulletin
2010;26(1):36-39
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study of the expression and distribution of four α-synuclein truncations in three cells.Method Four α-synuclein gene truncations were obtained by PCR method,followed by subcloning into the pEGFP-N1 eukaryotic expression vector.Four obtained recombination plasmids were transfected into MN9D cells,PC12 cells and SH-SY5Y cells using Lipofectamine 2000 respectively.The expression and distribution of four α-synuclein truncations were observed by Confocal.Results Distribution of four α-synuclein truncations was discrepant obviously,the truncations,with more C terminal remained,were prone to emerging in nuclei.Conclusion Localization of α-synuclein protein in cells may be related to the C terminal,and the whole C terminal plays an important role in distribution of α-synuclein into nuclei.
