Increased protein level of α-synuclein in the midbrain of Parkinson's disease rat model
- VernacularTitle:帕金森病模型大鼠中脑α-突触核蛋白增加
- Author:
Jiandong SUN
;
Yuhe YUAN
;
Yan LIU
;
Xiaofeng WANG
;
Naihong CHEN
- Publication Type:Journal Article
- Keywords:
Parkinson's disease;
rotenone;
α-synuclein;
tyrosine hydroxylase;
open field test;
midbrain
- From:
Chinese Pharmacological Bulletin
2010;26(1):73-77
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:Aim To study the effect of rotenone on α-synuclein in rat midbrain of Parkinson's disease(PD).Methods Rats were subcutaneouly injected with chronic low dose rotenone(1.0 mg·kg~(-1)·d~(-1)).Movements within 5 minutes were evaluated in open field test.Tyrosine hydroxylase(TH)-positive neuronsin the midbrain were measured with immunofluorescence staining.α-SYN protein level in the midbrain was demonstrated with immunohistochemical staining and Western blot.Results Compared to the control group,latency time,crossing,rearing and rearing time were changed significantly(P<0.05,0.01)in the rotenone group,TH-positive neurons were reduced(P<0.05)and α-SYN protein level in the midbrain was increased(P<0.05).Conclusion Injection with rotenone can induce PD symptom,which may be correlated to α-SYN protein level in the midbrain.
