The Expression of TSLP and Its Relationship with the Number of Infiltrating Regulatory T Cells in Lung Cancer
10.3969/j.issn.1000-8179.2010.03.002
- VernacularTitle:TSLP在肺癌组织中的表达及其与调节性T细胞的相关性
- Author:
Hua ZHAO
;
Hui LI
;
Jinpu YU
;
Yanjun SU
;
Lili YANG
;
Feng WEI
;
Xiumei AN
;
Fang XU
;
Dongsheng YUE
;
Xiaoliang ZHAO
;
Xiubao REN
- Publication Type:Journal Article
- Keywords:
TSLP;
Regulatory T cells;
Foxp3;
Immune tolerance;
Lung cancer
- From:
Chinese Journal of Clinical Oncology
2010;37(3):126-130
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of TSLP in human lung cancer tissue and the correla-tion between TSLP expression and number of regulatory T cells (Tregs). Methods: The expression of TSLP mRNA and protein was detected in different pathological lesions of the lung by Q-RT-PCR and immunohisto-chemistry. Immunohistochemistry was used to detect Foxp3+ Tregs. The correlation of TSLP with the number of Tregs was analyzed. Results: TSLP gene was expressed in tumor tissues (n=37), latero-tumor tissues (n=29) and non-tumor lung tissues (n=24), without statistical difference (P=0.148). TSLP protein was expressed in the cytoplasm and was observeed in 69.57% of tumor tissues, 13.33% of benign lesions and 30.00% of non-tumor lung tissues, with a significant difference (P<0.05). The expression of TSLP protein was correlated with tumor size (P=0.000) and lymph node metastasis (P=0.018). The number of Tregs in TSLP positive group was more than that in TSLP negative group (P<0.05). Conclusion: The expression of TSLP in lung tu-mor tissues is increased and is correlated with the number of Tregs, indicating that TSLP could induce Treg to play an important role in tumor immunotolerance.
