Sulfonation of polyethersulfone sheets effects on adsorbability of beta 2-microglobulin: Whether the adsorbability changes with increased sulfonation degree?
10.3969/j.issn.1673-8225.2010.03.011
- VernacularTitle:聚醚砜膜片磺化前后对β_2-微球蛋白的吸附能力:膜吸附能力随磺化度增加而发生变化吗?
- Author:
Xingyu MA
;
Xiaoqing SUN
;
Liping CHENG
;
Shudong SUN
;
Yilun YUE
;
Jia HUANG
;
Huayi MAO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(3):424-428
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Dialysis-related amyloid may occur during long-term dialysis for patients with uraemia, of which the main evocator is β_2-microglobulin (β_22M); therefore, how to eliminate 132M from blood is always the focus of research. OBJECTIVE: To observe ability of removal of β_2-microglobulin (β_2M) from serum using two kinds of polyethersulfone (PES) membrane materials with various degrees of sulfonation.METHODS: These materials were incubated in radio-labeled β_2M (~(125)Ⅰ-β_2M) solution and human serum respectively at appointed time at 37 ℃, and then the amounts of ~(125)Ⅰ-β_2M and serumβ_2M adsorbed by materials were measured by radio immunoassay. RESULTS AND CONCLUSION: In the ~(125)Ⅰ-β_2M system, amounts of ~(125)Ⅰ-β_2M adsorbed by the materials decreased in the following sequence PES with high degree of sulfonation > PES with low degree of sulfonation > PES, whatever the source of PES was. In the serum system, amounts of β_2M adsorbed reached maximums at 30 minutes and the final adsorptions decreased in sequence of PES with high degree of sulfonation > PES with low degree of sulfonaUon > PES. Sulfonated PES removed β_2M more than PES did and the adsorption of β_2M increases with the increase in the degree of sulfonation. Its ability to remove significant amount of β_2M may result in less β_2M available for incorporation into amyloid. The use of sulfonated PES membranes may lessen the likelihood of development of dialysis-related amyloidosis, which remains a major source of morbidity for patients treated with long-term hemodialysis.
