Interleukin-1β induced transdifferentiation of renal tubular epithelial cells and its effect on the cytoskeleton
- VernacularTitle:白细胞介素1β诱导肾小管上皮细胞转分化及其对细胞骨架的影响
- Author:
Guanglan WANG
;
Shuang CHEN
;
Xinrui WANG
;
Yingai SHI
;
Lihong ZHANG
;
Xiaohui LIU
;
Shan WU
- Publication Type:Journal Article
- Keywords:
Interleukin-1β;
Transdifferentiation;
α-SMA;
β-actin;
α-tubulin
- From:
Chinese Journal of Immunology
2010;26(3):210-213
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of interleukin-1β (IL-1β) on epithelial-mesenchymal transition (EMT) and cytoskeleton rearrangement of renal tubular epithelial cells.Methods:Immortalized renal tubular epithelial cell line NRK52E was cultured in vitro with IL-1β (30 μg/L) for 3 days and 6 days,then the cell morphology was observed;The mRNA expressions of α-smooth muscle actin (α-SMA),cytoskeleton components β-actin and α-tubulin were semi-quantitative examined by RT-PCR.The protein expression of α-SMA and arrangements of β-actin and α-tubulin were assessed by immunofluorescent staining.Results:After induced by IL-1β for 3 days and 6 days in vitro,the mRNA and protein expression of α-SMA increased significantly compared with corresponding control cells (P<0.001),it prompted that NRK52E cells underwent EMT;At the same time,the cell morphology also changed,from a typical multilateral paving stone to fibroblast-like appearance,with multiple processes; Cytoskeletal protein β-actin mRNA expression was also slightly increased (P<0.05).The distributions and arrangements of β-actin protein were also changed,from cell membrane transferred to peri-nucleus and cytoplasm,moreover it formed fiber bundle-like structures.However,another cytoskeleton protein α-tubulin in IL-1β induced cells,neither it's mRNA expression nor it's distribution had significant differences compared with the control group.Conclusion:IL-1β can induce NRK52E cells undergoing EMT in vitro,cell morphology changes into fibroblast-like appearance with multiple processes,and also the cytoskeleton protein β-actin expression increases and rearrangement occurrs.However,there was no changes onα-tubulin.
