Effects of andrographolide on the expression of IL-18 related cytokines in human PBMC/PBM
- VernacularTitle:穿心莲内酯对人PBMC/PBM表达IL-18相关细胞因子的影响
- Author:
Wei WANG
;
Chunhong LIU
;
Jing WANG
;
Paola ITALIANI
;
Xun WANG
;
Diana BORASCHI
;
Shiping MA
;
Di QU
- Publication Type:Journal Article
- Keywords:
Andrographolide;
IL-18;
IL-18BP;
Monocyte
- From:
Chinese Journal of Immunology
2010;26(3):218-223
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of andrographolide on the expression of IL-18 related cytokines by peripheral blood monocytes.Methods:After treatment of andrographolide in different concentrations on LPS stimulated human peripheral blood mononuclear cells (PBMC) and LPS+IFN-γ/IL-4 activated magnetic bead-sorted monocytes (PBM),the transcription level of IL-18,IL-18BP,IL-18Rα and IL-18Rβ was detected by real-time RT-PCR;and secreted IL-18,IL-18BP and IL-1β,IL-1Rα by PBM was detected with ELISA.Results:Andrographolide regulated the transcription of IL-18 and IL-18BP in a dose-and time-dependent effect.Andrographolide up-regulated the transcription of IL-18BP in LPS stimulated PBMC,and increased the ratio of IL-18BP/IL-18.The ratio of IL-18BP/IL-18 rose from 9.60 to 214 in LPS+IFNγ activated PBM,and IL-1Rα/IL-1β declined from 9 200 to 6 520 in IL-4 activated PBM by andrographolide treatment.Conclusion:Andrographolide can regulate IL-18 related gene transcription and expression in activated peripheral blood monocytes,and inhibit the excessive expression of IL-18 during inflammation.
