CdTe Nanoparticles Labeled with Anti-Fluorethene-Antibody and Fluorescent Immunoassay of Fluoranthene in Water Samples
10.3724/SP.J.1096.2010.00385
- VernacularTitle:碲化镉纳米晶标记抗荧蒽抗体及其荧光免疫分析
- Author:
Qiyan YE
;
Huisheng ZHUANG
;
Qionge WANG
;
Jinyan ZHANG
;
Chun ZHOU
- Publication Type:Journal Article
- Keywords:
Cadmium telluride;
Fluoranthene;
Direct competitive;
Fluorescent immunoassay
- From:
Chinese Journal of Analytical Chemistry
2010;38(3):385-388
- CountryChina
- Language:Chinese
-
Abstract:
The labeled compounds, CdTe was combined with anti-fluoranthene antibody, had good dispersion and stability with the fluorescence intensity enhancing. A direct competitive fluorescent immunoassay with CdTe-anti-fluoranthene antibody to detect fluoranthene in water sample in the environment was developed. The result showed that fluoranthene can be determined in the concentration range from 0.1 μg/L to 1000 μg/L with a correlation coefficient of 0.9983, a sensitivity of (IC_(50)) of 12.4 μg/L and a detection limit (IC_(20)) of 13.1 ng/L. Trace environmental pollutant in environmental water samples were successfully determined with a good accuracy and suitability. The recovery was between 95.1% and 111.0%, with relative standard deviation less than 9%.
